TSC AGAR BASE (TRYPTOSE SULFITE CYCLOSERINE AGAR BASE) ISO 7937 ISO 14189

For the detection and enumeration of Clostridium perfringens

Applications
  • Detection / Isolation / Enumeration
  • Isolation CLOSTRIDIUM PERFRINGENS
Packaging

Suspend 42 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 44-47ºC and aseptically add two vials of the selective supplement Clostridium perfringens (Cat. 6020) reconstituted in 5 ml of sterile distilled water. If desired, 25 ml of Egg Yolk Emulsion (Cat. 5152) can be added (Not indicated in ISO 7937). Homogenize gently and dispense into Petri dishes.

Clostridium perfringens supplement (Cat. 6020)

(1 vial for 500 ml of the medium)

D-Cycloserine ……………………… 200 mg

 

Formula
  • Casein Peptone 15
  • Soy Peptone 5
  • Yeast Extract 5
  • Disodium Disulfite (Anhydrous) 1
  • Ferric Ammonium Citrate 1
  • Bacteriological Agar 15

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