KF STREPTOCOCCAL AGAR WITH BROMOCRESOL PURPLE

For the selective isolation and enumeration of fecal enterococci by direct culture or by membrane filtration

Applications
  • Selective isolation / Enumeration
  • ENTEROCOCCUS
Packaging

Suspend 76.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 10 minutes. Cool to 45-50ºC and aseptically add two vial of TTC 1% supplement (Cat. 6030), previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes.

Formula
  • Maltose 20
  • Peptone Mixture 10
  • Yeast Extract 10
  • Sodium Glycerophosphate 10
  • Sodium Chloride 5
  • Lactose 1
  • Sodium Azide 0.4
  • Bromocresol Purple 0.015
  • Bacteriological Agar 20

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