COLUMBIA AGAR BASE EUROPEAN PHARMACOPOEIA, USP (ISO 10272)

For the isolation and cultivation of fastidious microorganisms and the determination of hemolytic reactions

Applications
  • Hemolytic Activity
  • Media for general use
  • OTHER APPLICATIONS
Packaging

Suspend 42.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. The medium is generally enriched with sterile defibrinated blood (sheep, rabbit or horse), serum or some other material. Cool to 45-50ºC and aseptically add 5-10% sterile defibrinated blood, homogenize gently and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood.

 

Formula
  • Pancreatic Digest of Casein 10
  • Yeast Extract 5
  • Meat Peptic Digest 5
  • Sodium Chloride 5
  • Heart Pancreatic Digest 3
  • Maize Starch 1
  • Bacteriological Agar 13.5

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