For the selective isolation and cultivation of gonococci and Haemophilus species when used with hemoglobin and supplements


  • Cultive / Isolation
  • Isolation / Cultivation

Suspend 18 grams of the medium in 250 ml of distilled water to make a double-strength base. Mix well and allow to stand for 5 minutes. Heat with frequent agitation and boil for one minute. Sterilize in autoclave at 121°C for 15 minutes. Also, autoclave 250 ml of a 2% haemoglobin solution elaborated by gradually adding water to 5 grams of dry haemoglobin to obtain a uniform suspension, before exposing it to the autoclave’s heat. Cool both flasks to 50°C and aseptically add the haemoglobin solution to GC Agar Base and mix gently. Aseptically add Polyenrichment Supplement (Cat. 6011), previously reconstituted with 1 vial of Polyenrichment Restoring Solution (vial B). Mix carefully to avoid bubble formation. This completed medium is the general purpose Chocolate Agar. Pour into plates or tubes with screw caps. Allow tubes to solidify with a long slant.

  • Peptone Mixture 15
  • Sodium Chloride 5
  • Dipotassium Phosphate 4
  • Corn Starch 1
  • Monopotassium Phosphate 1
  • Bacteriological Agar 10


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