For the differentiation of enteric bacilli

  • Diferenciación

Suspend 14 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50ºC and aseptically add 15 ml of a 0.5% potassium cyanide solution (0.5 g per 100 ml distilled water)to each tube containing 10 ml of medium and close tightly.

If desired, to view growth better, aseptically add 1 vial of TTC 1% Supplement (Cat. 6030), which produces a pink color. Mix with care. Distribute 10 ml into tubes.

Caution: Take extreme care when handling cyanide solution. Do not pipette by mouth.

  • Disodium Phosphate 5.64
  • Sodium Chloride 5
  • Peptone Mixture 3
  • Monopotassium Phosphate 0.225


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