MULLER KAUFFMANN TETRATHIONATE BROTH BASE

For the selective enrichment of Salmonella from meats and other foods

Applications
  • Selective enrichment
  • SALMONELLA
Packaging

Suspend 82 grams of the medium in one liter of distilled water. Mix well and dissolve by shortly heating with frequent agitation and cool it quickly.AVOID OVERHEATING.DO NOT AUTOCLAVE. A sediment of Calcium carbonate will remain. Aseptically add 20 ml/l of iodine solution and 10ml/l of 0.1% Brilliant Green solution. Distribute in tubes or flasks after homogenizing the possible precipitate. Once added, DO NOT REHEAT. Use the medium on the same day it is produced.

* Preparation of the iodine and potassium iodide: 5 g of potassium iodide, 4 g of iodine, and 20 ml of distilled water.

Formula
  • Sodium Thiosulfate 40.7
  • Calcium Carbonate 25
  • Ox Bile 4.75
  • Sodium Chloride 4.5
  • Meat Peptone 4.5
  • Yeast Extract 1.8
  • Beef Extract 0.9

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