SULPHITE LACTOSE BROTH BASE ISO 7937

Used for the confirmation of Clostridium perfringens

Applications
  • Comfirmation
  • Isolation CLOSTRIDIUM PERFRINGENS
Packaging

Suspend 20.3 grams of the medium in one liter of distilled water. Heat stirring frequently and boil gently until complete dissolution of the medium. Distribute 8 ml in test tubes with Durham hoods. Sterilize in an autoclave at 121 ° C for 15 minutes. Add to each tube of 8 ml, 0.5 ml of a 1.2% solution of sodium disulfite and 0.5 ml ml of a 1% solution of ferroammonium citrate, previously sterilized.

The medium can be stored at 3 ° C ± 2 ° C for 4 weeks.

 

Formula
  • Enzymatic Digestion of Casein 5
  • Yeast Extract 2.5
  • Sodium Chloride 2.5
  • Lactose 10
  • L-Cysteine Hydrochloride 0.3

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