BRILLIANT GREEN TETRATHIONATE BILE BROTH EUROPEAN PHARMACOPEIA

For the enrichment of Salmonella spp in foods, water and faeces

Applications
  • Selective enrichment
  • SALMONELLA
Packaging

Suspend 63 grams of the medium in one liter of distilled water .Mix well and dissolve by heating with frequent agitation. Do not boil. Dispense into sterilized containers homogenizing the medium well enough to distribute the calcium carbonate. AVOID OVERHEATING. DO NOT AUTOCLAVE. The prepared medium should be stored at 2-8°C. The color is milky green with calcium carbonate precipitate.

The dehydrated medium should be homogeneous, free-flowing and cream with green tint in color. If there are any physical changes, discard the medium.

 

The growth of Proteus is inhibited by taking the pH to 6.5 or also by adding Novobiocin at 0.4%

 

 

 

 

Formula
  • Calcium Carbonate 20
  • Potassium Tetrathionate 20
  • Meat Peptone 8.6
  • Dehydrated Ox Bile 8
  • Sodium Chloride 6.4
  • Brilliant Green 0.07

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