LEGIONELLA BCYE AGAR BASE ISO 11731-2

Selective medium for the cultivation of Legionella

Applications
  • Cultivation
  • LEGIONELLA
Packaging

Suspend 12.5 grams of the medium in 450 ml of distilled water. Heat until boiling and until the medium is completely dissolved. Distribute into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. Cool to 45 - 50°C and aseptically add 5 vials of Legionella BCYE Growth Supplement (Cat. 6022), previously reconstituted in 10 ml of warm sterile distilled water each one, and if desired also add 1 vial of LEGIONELLA GVPC SUPPLEMENT (Cat. 6025), previously reconstituted with sterile distilled or deionized water to about 80% of the volume (see Preparation of Cat. 6025). Mix well and distribute into appropriate containers.

Formula
  • Yeast Extract 10
  • Activated Charcoal 2
  • Bacteriological Agar 13

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