BLOOD AGAR BASE Nº 2 (ISO 7932)

For the cultivation and detection of hemolytic activity of fastidious microorganisms, confirmation of Bacillus cereus(ISO 7932)and Listeria monocytogenes (ISO 11290-19)

Applications
  • Hemolytic Activity
  • Confirmation
  • LISTERIA
  • OTHER APPLICATIONS
Packaging

Suspend 39.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 5-7% of sterile defibrinated blood, homogenize and pour intro Petri dishes.

Formula
  • Proteose Peptone 15
  • Yeast Extract 5
  • Sodium Chloride 5
  • Liver Extract 2.5
  • Bacteriological Agar 12

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