BURKHOLDERIA CEPACIA AGAR BASE

For the selective isolation of Burkholderia cepacia from respiratory secretions of Cystic Fibrosis patients, and for the routine testing of non-sterile inorganic salts containing preservatives

Applications
  • Selective Isolation
  • BURKHOLDERIA CEPACIA
Packaging

Suspend 18.25 grams of the medium in 500ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Burkholderia Selective Supplement (Cat. 6032), previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes.

Formula
  • Sodium Pyruvate 7
  • Peptone 5
  • Potassium Dihydrogen Phosphate 4.4
  • Yeast extract 4
  • Bile Salts 1.5
  • Disodium Hydrogen Phosphate 1.4
  • Ammonium Sulphate 1
  • Magnesium Sulphate 0.2
  • Phenol Red 0.02
  • Ferrous Ammonium Sulphate 0.01
  • Crystal Violet 0.001
  • Bacteriological Agar 12

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