Standard medium for the detection of antimicrobial inhibitors in meat and organ samples with Bacillus subtilis and Micrococcus luteus spores as test organisms

  • Antibiotic Assay

Suspend 27.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add a spore suspension of Bacillus subtilis (BGA) containing approximately 1x10^7 cfu/ml (final concentration in mediim 1x10^4 cfu/ml). If desired, add a suspension of Micrococcus luteus ATCC 9341 suspension. Pour into plates. The prepared medium should be used immediately.

  • Sodium Chloride 5.1
  • Casein Peptone 3.45
  • Meat Peptone 3.45
  • Trisodium Phosphate 12-hydrate 2.4
  • Bacteriological Agar 13


Join us at Medica 2018| Düsseldorf, Germany

We would like to invite you to visit us at stand 18C24 in Hall 18, from 12 - 15 November 2018, where we will be happy to review next years strategies and provide information of all our products,...

MEDICA 2017: Once again Conda will be in Düsseldorf

Laboratorios Conda is pleased to announce the attendance to MEDICA 2017 in Düsseldorf, Germany (13-16th november). We´ll be showcasing our latest products, planning new strategies and...

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