MIDDLEBROOK BROTH

For the isolation and cultivation of Micobacteria, used with the ENRICHEMENT ADC SUPPLEMENT

Applications
  • Isolation
  • Cultivation
  • MYCOBACTERIA
Packaging

Suspend 4.7 grams of the medium in 900 ml of distilled water. Add 2 ml of Glycerol. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one 100 ml flask of the enrichment supplement ADC (Cat. 6038). Homogenize gently.

Formula
  • Disodium Phosphate 2.5
  • Monopotassium Phosphate 1
  • Ammonium Sulfate 0.5
  • L-Glutamic Acid 0.5
  • Sodium Citrate 0.1
  • Magnessium Sulfate 0.05
  • Ferricammonium Citrate 0.04
  • Pyridoxine 1
  • Zinc Sulfate 1
  • Cooper Sulfate 1
  • Biotin 0.5
  • Calcium Chloride 0.5

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