TRYPTICASEIN SOY AGAR (TSA) EUROPEAN PHARMACOPOEIA, USP

For general bacteriology methods and the determination of hemolytic reactions

Applications
  • Plate count media
  • Media for general use
  • OTHER APPLICATIONS
Packaging

Suspend 40 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Large quantities may require a longer sterilization time, but the temperature should not be increased. To prepare blood plates for hemolysis studies, add 5-10% of defibrinated sterile blood to the sterile medium, previously cooled to 45°C. Be careful to avoid bubble formation when adding the blood to the cooled medium and rotate the flask or bottle slowly to create a homogeneous solution.

Formula
  • Pancreatic Digest of Casein 15
  • Papaic Digest of Soya Bean 5
  • Sodium Chloride 5
  • Bacteriological Agar 15

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