For the detection and isolation of Bordetella pertussis and Bordetella parapertussis from clinical samples



  • Detection / Isolation

Suspend 36 grams of the medium in one liter of distilled water with 10 ml of glycerol. Allow to stand for 5 minutes and mix well until a uniform suspension is obtained. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 15-20% of sterile defibrinated horse blood, homogenize and pour into Petri dishes. The medium can be made more selective by aseptically adding 2 vials of Bordetella Supplement (Cat. 6015), previously reconstituted in 5 ml of sterile distilled water.

  • Proteose Peptone 10
  • Sodium Chloride 5.5
  • Potato Infusion 4.5
  • Bacteriological Agar 16


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