UREA AGAR BASE (CHRISTENSEN) ISO 6579, ISO 19250

For the differentiation of Enterobacteria on the basis of urease production

Applications
  • Identification
  • Diferenciación
  • ENTEROBACTERIA
  • SALMONELLA
Packaging

Dissolve 29 grams of the medium in 100 ml of distilled water. Sterilize by filtration. Separately dissolve 15 grams of agar in 900 ml of distilled water by boiling. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 50ºC and add to the 100 ml of the sterile Urea Agar Base. Mix well and dispense aseptically in sterile tubes. Leave the medium to set in a slanted position so as to obtain deep butts. Do not remelt the slanted agar.

Formula
  • Urea 20
  • Sodium Chloride 5
  • Monopotassium Phosphate 2
  • Gelatin Peptone 1
  • Dextrose 1
  • Phenol Red 0.012

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