YERSINIA SELECTIVE AGAR BASE ISO 10273

Selective medium for Yersinia enterocolitica used with supplement

Applications
  • Isolation
  • YERSINIA
Packaging

Suspend 29 grams of the medium in 500 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Yersinia selective supplement (Cat. 6033), previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes.

 

Yersinia Selective Supplement (CIN) Cat. 6033

(Composition: each vial for 500 ml)

 

Cefsulodin…………………………………..….7.5 mg

Irgasan………………………………….……...2.0 mg

Novobiocine…………………..…………….1.25 mg

 

Formula
  • D-Mannitol 20
  • Enzymatic Digest of Gelatin 17
  • Enzymatic Digest of Casein and Animal Tissues 3
  • Yeast Extract 2
  • Sodium Pyruvate 2
  • Sodium Chloride 1
  • Sodium Desoxycholate 0.5
  • Neutral Red 0.03
  • Magnesium Sulfate Anhydrous 0.01
  • Crystal Violet 0.001
  • Bacteriological Agar 12.5

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