T.S.A AGAR Nº 2 MODIFIED

For the isolation, cultivation and detection of hemolytic activity of fastidious microorganisms

Applications
  • Media for general use
  • OTHER APPLICATIONS
Packaging

Suspend 44.5 grams of the medium in one liter of distilled water. Mix well until a uniform suspension is obtained. Heat with gentle agitation and boil for one minute. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50°C, and aseptically add 7% sterile defibrinated sheep blood. Homogenize and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood to the cooled medium and rotate the flask or bottle slowly to create a homogeneous solution.

Formula
  • Tryptone H 15
  • Soy Peptone 5
  • Sodium Chloride 5
  • Growth Factors 4.5
  • Bacteriological Agar 15

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