For the isolation of Clostridium difficile when used with supplements

  • Isolation

Suspend 34.5 grams of the medium in 500ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Clostridium Difficile Supplement (Cat. 6061), previously reconstituted in 5 ml of sterile distilled water and 7% sterile defibrinated horse blood. Homogenize gently and dispense into Petri dishes. Be careful to avoid bubble formation when adding the blood and rotate the flask or bottle slowly to create a homogeneous solution.

  • Proteose Peptone 40
  • Fructose 6
  • Disodium Hydrogen Phosphate 5
  • Potassium Dihydrogen Phosphate 1
  • Magnesium sulfate 0.1
  • Sodium Chloride 2
  • Bacteriological Agar 15


Join us at Medica 2018| Düsseldorf, Germany

We would like to invite you to visit us at stand 18C24 in Hall 18, from 12 - 15 November 2018, where we will be happy to review next years strategies and provide information of all our products,...

MEDICA 2017: Once again Conda will be in Düsseldorf

Laboratorios Conda is pleased to announce the attendance to MEDICA 2017 in Düsseldorf, Germany (13-16th november). We´ll be showcasing our latest products, planning new strategies and...

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