CLOSTRIDIUM DIFFICILE AGAR BASE

For the isolation of Clostridium difficile when used with supplements

Applications
  • Isolation
  • CLOSTRIDIUM DIFFICILE
Packaging

Suspend 34.5 grams of the medium in 500ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Clostridium Difficile Supplement (Cat. 6061), previously reconstituted in 5 ml of sterile distilled water and 7% sterile defibrinated horse blood. Homogenize gently and dispense into Petri dishes. Be careful to avoid bubble formation when adding the blood and rotate the flask or bottle slowly to create a homogeneous solution.

Formula
  • Proteose Peptone 40
  • Fructose 6
  • Disodium Hydrogen Phosphate 5
  • Potassium Dihydrogen Phosphate 1
  • Magnesium sulfate 0.1
  • Sodium Chloride 2
  • Bacteriological Agar 15

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International Sales Manager (Italy)

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