Generate UMI-labeled, stranded libraries from pico-input for whole-transcriptome analyses,compatible with Illumina NGS platforms
RNA-seq can be challenging in some situations, including cases where input amounts are low or comprised of degraded RNA samples. Takara Bio was a pioneer in the development of a low-input solutions with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian which allows researchers to be successful with lower inputs.
Topics discussed:
Overview of the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian now features the inclusion of unique molecular identifiers (UMIs) to help mitigate PCR bias and provide the user with the opportunity to identify true variance within the sample.