Published: 09/05/24 11:39 Categories: Microbiology

Peptones are the most widely used source of organic nitrogen for the formulation of microbiological media, whether for detection, quantification, or biotechnological applications. Among the wide range of peptones available, today we want to talk about Proteose Peptone No. 3. 


This hydrolysate is defined as an enzymatic digest of proteins from various animal sources. 


Peptones in general began to gain popularity at the end of the 19th century, but it was during World War I when the first Proteose Peptone No. 3 was developed. Its development focused on finding an ingredient that would generate high toxin production in cultures of Corynebacterium diphtheriae, thereby synthesizing the vaccine against diphtheria. 



Vaccines and diagnostics 


As it was conceived for the synthesis of vaccines, Proteose Peptone No. 3 is a hydrolysate with a strong presence in pharmaceutical industry. It is currently widely used to produce multivalent bacterial and veterinary vaccines. However, its nutritional richness has made it a component in culture media for the detection and identification of multiple organisms; some examples are: 

  • R2A Agar, used for counting aerobes in treated waters. 
  • MRS Broth, with applications such as culturing and enumeration of Lactobacillus or for the manufacture of probiotics. 
  • Granada Agar Base, a selective and differential medium for detecting Streptococcus agalactiae in clinical samples. 


High concentration of peptides and amino acids 

Its wide array of ingredients means that it is not necessary to mix it with other peptones, although it creates a synergistic effect with yeast extract. Within the peptide profile, the values of glutamic acid, proline, and leucine stand out. 



Proteose Peptone No. 3 is one of the most complete bioingredients, being the first choice for countless fermentations and diagnostic media. 


If you want to consult more information about Proteose Peptone No. 3 from Condalab, follow the link to our website, and to learn more about the peptide profiles of our peptone catalog, download our bioprocess catalog.